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KMID : 0364820160520030383
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Korean Journal of Microbiology
2016 Volume.52 No. 3 p.383 ~ p.387
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Effects of Cdc31, a component of TREX-2 complex, on growth and mRNA export in fission yeast
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Koh Eun-Jin
Yoon Jin-Ho
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Abstract
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In fission yeast, Schizosaccharomyces pombe, the cdc31 gene encodes a member of the conserved Ca2+-binding centrin/ CDC31 family, which is a component of spindle pole body. Here, we demonstrate that the S. pombe cdc31p is also a component of TREX-2 complex, which influences mRNA export from the nucleus to the cytoplasm. Repression of the cdc31 gene expression caused growth defect with accumulation of poly(A)+ RNA in the nucleus. On the other hand, over-expression of cdc31 exhibited no defects of both growth and bulk mRNA export, but showed somewhat longer cell morphology. Yeast two-hybrid analysis showed that Cdc31 interacted with Sac3 and Pci2, the subunits of TREX-2 complex. These results suggest that S. pombe Cdc31 is also involved in mRNA export as a component of TREX-2 complex.
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KEYWORD
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cdc31, fission yeast S. pombe, mRNA export, TREX-2 complex
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